In the present study, 'natural C-13 tracer method' was used to partition the belowground respiration into rhizosphere respiration and soil microbial respiration to test the hypothesis that defoliation affects rhizosphere respiration and rhizosphere priming effect on decomposition of soil organic matter (SOM). A C-3 plant species, Helianthus annuus (sunflower), was grown in 'C-4' soil in microcosms so that the CO2 evolved from plant-soil system can be partitioned. Four levels of defoliation intensities were established by manual clipping. CO2 evolved from plant-soil system was trapped during 0-4 h after defoliation (HAD), 5-22 HAD and 23-46 HAD using a closed circulating system, respectively. We found that both rhizosphere respiration and soil microbial respiration of the clipped plants were either unchanged or significantly enhanced compared to unclipped plants at 45% defoliation level during all sampling intervals. Soil microbial respiration increased significantly at all defoliation levels during 0-4 HAD, however, both rhizosphere respiration and soil microbial respiration decreased significantly during 5-22 HAD or 23-46 HAD when 20% or 74 clearly demonstrated that the defoliation treatments modified the rhizosphere priming effect on SOM decomposition. The total cumulative rhizosphere priming effects on SOM decomposition during 0-46 HAD were 146%,241%,204% and 205% when 0%, 20%,45% and <74%.